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Published Papers from the Nanomedicine Center


Biggs MP, Santos L, Milone M, Gondarenko A, Wind SJ.

High Resolution Imaging of the Immunological Synapse and TCR Microclustering through Microfabricated Substrates.

Journal of the Royal Society Interface. 2010; ASAP articles.

Grupp SA, June CH.

Adoptive cellular therapy.

Curr Top Microbiol Immunol . 2011;344:149-72.

Palma M, Abramson JJ, Gorodetsky AA, Penzo E, Gonzalez RL Jr, Sheetz MP, Nuckolls C, Hone J, Wind SJ.

Selective biomolecular nanoarrays for parallel single-molecule investigations.

J Am Chem Soc 2011; 133(20):7656-9.

Porat Z, Yaron I, Katz BZ, Kam Z, Geiger B.

Shear flow-induced formation of tubular cell protusions in multiple myeloma cells.

J Cell Physiol. 2011; [Epub ahead of print].

Schvartzman M, Palma M, Sable J, Abramsom J, Hu X, Sheetz MP, Wind SJ.

Nanolithographic control of the spatial organization of cellular adhesion receptors at the single-molecule level.

Nano Lett. . 2011;11(3):1306-12.

Wolfenson H, Bershadsky AD, Henis Y, Geiger B.

Actomyosin-generated tension controls the molecular kinetics of focal adhesions.

J Cell Science. 2011;124:1425-32.


Bronson JE, Hofman JM, Fei J, Gonzalez Jr RL and Wiggins CH.

Graphical models for inferring single molecule dynamics.

BMC Bioinformatics. 2010;11.

Fardin MA, Rossier OM, Rangamani P, Avigan PD, Gauthier NC, Vonnegut W, Mathur A, Hone J, Iyengar R, Sheetz MP.

Cell spreading as a hydrodynamic process.

Soft Matter. 2010;6:4788.

Greenfield A, Madar A, Ostrer H, Bonneau R.

DREAM4: Combining Genetic and Dynamic Information to Identify Biological Networks and Dynamical Models.

PLoS ONE. 2010; 5(10); e13397.

Mann M, Barad O, Agami R, Geiger B and Hornstein E.

miRNA-based mechanism for the commitment of multipotent progenitors to a single cellular fate.

Proc Natl Acad Sci U S A. 2010;107(36):15804-9.

Paulos CM, Carpenito C, Plesa G, Suhoski MM, Varela-Rohena A, Golovina TN, Carroll RG, Riley JL, June CH.

The inducible costimulator ICOS is critical for the development of human TH17 cells.

Science Translational Medicine. 2010; 2; 55-78.

Rolli CG, Seufferlein T, Kemkemer R, Spatz JP.

Impact of tumor cell cytoskeleton organization on invasiveness and migration: a microchannel-based approach.

PLoS ONE. 2010;5(1):e8726.

Rossier OM, Gauthier N, Biais N, Vonnegut W, Fardin MA, Avigan P, et al.

Force generated by actomyosin contraction builds bridges between adhesive contacts.

Embo J. 2010;29(6):1055-68.

Schoen I, Hu W, Klotzsch E, Vogel V.

Probing Cellular Traction Forces by Micropillar Arrays: Contribution of Substrate Warping to Pillar Deflection.

Nanoletters. 2010;10:1823-1830.

Shen K, Milone MC, Dustin ML, Kam LC.

Nanoengineering of Immune Cell Function.

MRS Proceedings, 2010; 1209. pii: 1209-YY03-01.

Shi P, Nedelec S, Wichterle H, Kam LC.

Combined microfluidics/protein patterning platform for pharmacological interrogation of axon pathfinding.

Lab Chip . 2010;10(8):1005-10.

Tsai J, Kam LC.

Lateral Mobility of E-cadherin Enhances Rac1 Response in Epithelial Cells.

Cell Mol Bioeng . 2010;3(1):84-90.

Vardhana S, Choudhuri K, Varma R, Dustin ML.

Essential role of Ubiquitin and TSG101 in formation and function of the central supramolecular activation cluster.

Immunity. 2010;32(4):531-40.

Waltman P, Kacmarczyk T, Bate AR, Kearns DB, Reiss DJ, Eichenberger P, Bonneau R.

Multi-species integrative biclustering.

Genome Biol. 2010;11(9):R96. Epub 2010 Sep 29.

Zaidel-Bar R and Geiger B.

The switchable integrin adhesome.

J Cell Sci. 2010;123(Pt 9): 1385-8.

Zanin-Zhorov A, Ding Y, Kumari S, Attur M, Hippen K, Brown M, et al.

Protein kinase C-theta mediates negative feedback on regulatory T cell function.

Science. 2010;328(5976):372-6.

Zhao Y, Moon E, Carpenito C, Paulos CM, Liu X, Brennan A, Chew A, Carroll RG, Scholler J, Levine BL, Albelda SM, June CH.

Multiple injections of electroporated autologous T cells expressing a chimeric antigen receptor mediate regression of human disseminated tumor.

Canc Res. 2010; 70(22):9053-61.


Bronson JE, Fei J, Hofman JM, Gonzalez RL, Jr., Wiggins CH.

Learning rates and states from biophysical time series: a Bayesian approach to model selection and single-molecule FRET data.

Biophys J. 2009;97(12):3196-205.

del Rio A, Perez-Jimenez R, Liu R, Roca-Cusachs P, Fernandez JM, Sheetz MP.

Stretching single talin rod molecules activates vinculin binding.

Science. 2009;323(5914):638-41.

Dustin ML.

The cellular context of T cell signaling.

Immunity. 2009;30(4):482-92.

Fei J, Bronson JE, Hofman JM, Srinivas RL, Wiggins CH, Gonzalez RL, Jr.

Allosteric collaboration between elongation factor G and the ribosomal L1 stalk directs tRNA movements during translation.

Proc Natl Acad Sci U S A. 2009;106(37):15702-7.

Ghassemi S, Rossier, O., Sheetz, M. P., Wind, S. J., Hone, J.

Gold-tipped elastomeric pillars for cellular mechanotransduction.

Journal of Vacuum Science & Technology 2009(B 27):3088-91

Goldyn AM, Rioja BA, Spatz JP, Ballestrem C, Kemkemer R.

Force-induced cell polarisation is linked to RhoA-driven microtubule-independent focal-adhesion sliding.

J Cell Sci. 2009;122(Pt 20):3644-51.

Hosseini BH, Louban I, Djandji D, Wabnitz GH, Deeg J, Bulbuc N, et al.

Immune synapse formation determines interaction forces between T cells and antigen-presenting cells measured by atomic force microscopy.

Proc Natl Acad Sci U S A. 2009;106(42):17852-7.

Huang J, Grater SV, Corbellini F, Rinck S, Bock E, Kemkemer R, et al.

Impact of order and disorder in RGD nanopatterns on cell adhesion.

Nano Lett. 2009;9(3):1111-6.

Ilani T, Vasiliver-Shamis G, Vardhana S, Bretscher A, Dustin ML.

T cell antigen receptor signaling and immunological synapse stability require myosin IIA.

Nat Immunol. 2009;10(5):531-9.

Klotzsch E, Smith ML, Kubow KE, Muntwyler S, Little WC, Beyeler F, et al.

Fibronectin forms the most extensible biological fibers displaying switchable force-exposed cryptic binding sites.

Proc Natl Acad Sci U S A. 2009;106(43):18267-72.

Kubow KE, Klotzsch E, Smith ML, Gourdon D, Little WC, Vogel V.

Crosslinking of cell-derived 3D scaffolds up-regulates the stretching and unfolding of new extracellular matrix assembled by reseeded cells.

Integr Biol (Camb). 2009;1(11-12):635-48.

Little WC, Schwartlander R, Smith ML, Gourdon D, Vogel V.

Stretched extracellular matrix proteins turn fouling and are functionally rescued by the chaperones albumin and casein.

Nano Lett. 2009;9(12):4158-67.

Moore SW, Biais N, Sheetz MP.

Traction on immobilized netrin-1 is sufficient to reorient axons.

Science. 2009;325(5937):166.

Milone MC, Fish JD, Carpenito C, Carroll RG, Binder GK, Teachey D, et al.

Chimeric receptors containing CD137 signal transduction domains mediate enhanced survival of T cells and increased antileukemic efficacy in vivo.

Mol Ther. 2009;17(8):1453-64.

Mugler A, Walczak AM, Wiggins CH.

Spectral solutions to stochastic models of gene expression with bursts and regulation.

Phys Rev E Stat Nonlin Soft Matter Phys. 2009;80(4 Pt 1):041921.

Puklin-Faucher E, Sheetz MP.

The mechanical integrin cycle.

J Cell Sci. 2009;122(Pt 2):179-86. PMCID

Puklin-Faucher E, Vogel V.

Integrin activation dynamics between the RGD-binding site and the headpiece hinge.

J Biol Chem. 2009;284(52):36557-68.

Ranzinger J, Krippner-Heidenreich A, Haraszti T, Bock E, Tepperink J, Spatz JP, et al.

Nanoscale arrangement of apoptotic ligands reveals a demand for a minimal lateral distance for efficient death receptor activation.

Nano Lett. 2009;9(12):4240-5.

Roca-Cusachs P, Gauthier NC, Del Rio A, Sheetz MP.

Clustering of alpha(5)beta(1) integrins determines adhesion strength whereas alpha(v)beta(3) and talin enable mechanotransduction.

Proc Natl Acad Sci U S A. 2009;106(38):16245-50.

Schvartzman M, Nguyen K, Palma M, Abramson J, Sable J, Hone J, et al.

Fabrication of Nanoscale Bioarrays for the Study of Cytoskeletal Protein Binding Interactions Using Nanoimprint Lithography.

J Vac Sci Technol B Microelectron Nanometer Struct Process Meas Phenom. 2009;27(1):61-5.

Schvartzman M, Wind SJ.

Robust pattern transfer of nanoimprinted features for sub-5-nm fabrication.

Nano Lett. 2009;9(10):3629-34.

Schvartzman M, Wind SJ.

Plasma fluorination of diamond-like carbon surfaces: mechanism and application to nanoimprint lithography.

Nanotechnology. 2009;20(14):145306.

Shen K, Tsai J, Shi P, Kam LC.

Self-aligned supported lipid bilayers for patterning the cell-substrate interface.

J Am Chem Soc. 2009;131(37):13204-5.

Shi P, Shen K, Chassemi S, Hone J, Kam LC.

Dynamic force generation by neural stem cells.

Cellular and Molecular Bioengineering. 2009;2:464.

Tsai J, Kam L.

Rigidity-dependent cross talk between integrin and cadherin signaling.

Biophys J. 2009;96(6):L39-41.

Vasiliver-Shamis G, Cho MW, Hioe CE, Dustin ML.

Human immunodeficiency virus type 1 envelope gp120-induced partial T cell receptor signaling creates an F-actin-depleted zone in the virological synapse.

J Virol. 2009;83(21):11341-55.

Walczak AM, Mugler A, Wiggins CH.

A stochastic spectral analysis of transcriptional regulatory cascades.

Proc Natl Acad Sci U S A. 2009;106(16):6529-34.



Antia M, Baneyx G, Kubow KE, Young G, Vogel V.

Fibronectin in aging extracellular matrix fibrils is increasingly unfolded by cells and elicits an enhanced rigidity response.

Faraday Discussions 2008 139:229 - 249.

Dubin-Thaler BJ, Hofman JM, Cai Y, Xenias H, Spielman I, Shneidman AV, David LA, Dobereiner HG, Wiggins CH, and MP Sheetz.

Quantification of cell edge velocities and traction forces reveals distinct motility modules during cell spreadingd by cells.

PLoS ONE 2008 3:e3735.

Dunlop I, Zorn S, Richter G, Srot V, Kelsch M, Van Aken PA, Skoda M, Gerlach A, Spatz JP, and F Schreiber.

Titanium-silicon oxide film structures for polarization-modulated infrared reflection absorption spectroscopy.

Thin Solid Films 2008:517(6):2048-2054.

Ghassemi S, Biais N, Maniura K, Wind SJ, Sheetz MP, and J Hone.

Fabrication of elastomer pillar arrays with modulated stiffness for cellular force measurements.

J. Vac. Sci. Technol. B. 2008 26:2549-2533.

Hirschfeld-Warneken VC, Arnold M, Cavalcanti-Adam A, Lopez-Garcia M, Kessler H, and JP Spatz.

Cell adhesion and polarisation on molecularly defined spacing gradient surfaces of cyclic RGDfK peptide patches.

Eur J Cell Biol. 2008 87:743-50.

Hofman JM, and CH Wiggins.

Bayesian Approach to Network Modularity.

Physical Review Letters. 2008 100:258701.

Huang, X. et al., Sleeping Beauty transposon-mediated engineering of human primary T cells for therapy of CD19+ lymphoid malignancies. Mol Ther 16 (3), 580 (2008)

Hytönen VP, Vogel V.

How force might activate talin’s vinculin binding sites: SMD reveals a structural mechanism.

PLoS Computational Biol 2008 4(2):e24.

Jungbauer, S., H. Gao, J.P. Spatz, and R. Kemkemer.

Two characteristic regimes in frequency dependent dynamic reorientation of fibroblasts on cyclically stretched substrates.

Biophys J 2008 95:3470-8..

Little W, Smith ML, Ebneter U, Vogel V.

Assay to mechanically tune and optically probe fibrillar fibronectin conformations from fully relaxed to breakage.

Matrix Biol. 2008 27(5), 451-461.

Neves SR, Tsokas P, Sarkar A, Grace EA, Rangamani P, Taubenfeld SM, Alberini CM, Schaff JC, Blitzer RD, Moraru II, Iyengar R.

Cell shape and negative links in regulatory motifs together control spatial information flow in signaling networks.

Cell 2008 133(4), 666-680.

Schvartzman M, Mathur A, Hone J, Jahnes C, and SJ Wind.

Plasma fluorination of carbon-based materials for imprint and molding lithographic applications.

Applied Physics Letters. 2008. 93:153105.

Schvartzman M, Mathur A, Kang Y, Jahnes C, Hone J, and SJ Wind.

Fluorinated diamondlike carbon templates for high resolution nanoimprint lithography.

J. Vac. Sci. Technol. B. 2008 26:2394-2398.

Selhuber-Unkel C, Lopez-Garcia M, Kessler H, and JP Spatz.

Cooperativity in Adhesion Cluster Formation during Initial Cell Adhesion.

Biophys J. 2008 95(11):5424-31.

Shen K, Thomas VK, Dustin ML, Kam L.

Micropatterning of costimulatory ligands enhances CD4+ T cell function.

PNAS 2008 105(22), 7791-7796.

Tsai J, Sun E, Gao Y, Hone JC, Kam LC.

Non-brownian diffusion of membrane molecules in nanopatterned supported lipid bilayers.

Nano Letters 2008 8, 425-430.

Ulmer J, Geiger B, and JP Spatz.

Force-induced fibronectin fibrillogenesis in vitro.

Soft Matter. 4:1998-2007.

Visnapuu, M. L., Fazio, T., Wind, S. & Greene, E. C. , Parallel arrays of geometric nanowells for assembling curtains of DNA with controlled lateral dispersion. . Langmuir (24), 11293 (2008)

Zhang X, Jiang G, Cai Y, Monkley SJ, Critchley DR, and MP Sheetz.

Talin depletion reveals independence of initial cell spreading from integrin activation and traction.

Nat Cell Biol. 2008 10(9):1062-1068.


Blummel J, Perschmann N, Aydin D, Drinjakovic J, Surrey T, Lopez-Garcia M, Kessler H, Spatz J.P.

Protein repellent properties of covalently attached PEG coatings on nanostructured SiO2-based interfaces.

Biomaterials 2007 28, 4739-4747.

Cavalcanti-Adam EA, Volberg T, Micoulet A, Kessler H, Geiger B, Spatz J.
Cell Spreading and Focal Adhesion Dynamics Are Regulated by Spacing of Integrin Ligands.
Biophysical J. 2007 92(8), 2964-2974.

Christian, N. A. et al., Tat-functionalized near-infrared emissive polymersomes for dendritic cell labeling. Bioconjug Chem 18 (1), 31 (2007).

Giannone G, Dubin-Thaler B, Rossier O, Cai Y, Chaga O, Jiang G, Beaver W, Doebereiner HG, Freund Y, Borisy G, Sheetz MP.

Lamellipodial Actin Mechanically Links Myosin Activity with Adhesion-Site Formation.
Cell 2007 128, 561-575.

Sims T, Soos T, Xenias H, Dubin-Thaler B, Hofman J, Waite J, Cameron T, Thomas VK, Varma R, Wiggins C, Sheetz MP, Littman D, Dustin ML.
Opposing Effects of PKCq and WASP on Symmetry Breaking and Relocation of the Immunological Synapse.
Cell 2007 129, 773-785.

Smith ML, Gourdon D, Little WC, Kubow KE, Eguiluz RA, Luna-Morris S, Vogel V.
Force-induced unfolding of fibronectin in the extracellular matrix of living cells.
PLoS Biology 2007 5(10), e268.

Storrie H, Guler M, Abu-Amara S, Volberg T, Rao M, Geiger B, Stupp S.
Supramolecular crafting of cell adhesion.
Biomaterials 2007 28(31), 4608-4618

Suhoski, M. M. et al., Engineering artificial antigen-presenting cells to express a diverse array of co-stimulatory molecules. Mol Ther 15 (5), 981 (2007).

Zaidel-Bar R, Itzkovitz S, Ma’ayan A, Iyengar R, Geiger B.
Functional atlas of the integrin adhesome.
Nature Cell Biology 2007 9(8), 858-867.


Cai Y, Biais N, Giannone G, Tanase M, Ladoux B, Hofman J, Wiggins CH, Sheetz MP.
Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow.
Biophysical J. 2006 91(10), 3907-3920

Doebereiner HG, Dubin-Thaler BJ, Hofman JM, Xenias HS, Sims TN, Giannone G, Dustin MI, Wiggins C, Sheetz MP.
Lateral membrane waves constitute a universal dynamic pattern of motile cells.
Physical Review Letters. 2006 97(3):038102.

Sawada Y, Tamada M, Dubin-Thaler B, Cherniavskaya O, Sakai R, Tanaka S, Sheetz MP.
Force Sensing by Mechanical Extension of the Src Family Kinase Substrate p130Cas.
Cell 2006 127, 1015-1026.

Vogel V, Sheetz MP.
Local force and geometry sensing regulate cell functions.
Nature Review Molecular Cell Biology. 2006 7(4), 265-275.


Milone, M. C. et al., Treatment of primary Epstein-Barr virus infection in patients with X-linked lymphoproliferative disease using B-cell-directed therapy. Blood 105 (3), 994 (2005).


Dunlop, I., M.L. Dustin and J.P. Spatz.  2008. Artificial biomimetic substrates in the study of the immunological synapse.

In Nanotechnology (Major Reference Work).

Editors: H. Fuchs, M. Grätzel, H. Krug, G. Schmid, V. Vogel, and R. Waser, Wiley-VCH Verlag GmbH & Co, Germany. (in press).

Hytönen, V.P., M.L. Smith, and V. Vogel. 2008. Translating mechanical force into discrete biochemical signal changes: multimodularity imposes unique properties to mechanotransductive proteins.

In Mechanotransduction.

Editor: Mohammad Mofrad. (in press).

Vogel, V., and M. P. Sheetz. 2008.

A Matter of Force in Health and Disease: engineering cell and tissue functions by controlling mechanical aspects.

In Nanomedicine.

Editor: Viola Vogel, Wiley. (in press).

Tanase, M., N. Biais, and M. P. Sheetz. 2007. Magnetic tweezers in Cell Biology.

In Methods in Cell Biology.

Editors: D. Discher, and Y. L. Wang, AP.

Published Papers of Mechanical Biology Studies

Engler AJ, Sen S, Sweeney HL, Discher DE.
Matrix elasticity directs stem cell lineage specification.
Cell. 2006;126(4):677-689.

Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE.
Geometric control of cell life and death.
Science. 1997;276(5317):1425-1428.

Sawada Y, Sheetz MP.
Force Transduction by Triton Cytoskeletons.
J. Cell Biol. 2002;156:609-615.

Choquet D, DP Felsenfeld, MP Sheetz.
Extracellular matrix rigidity causes strengthening of integrin-cytoskeletal linkages.
Cell. 1997;88:39-48.

Kam L, Boxer SG.
Cell adhesion to protein-micropatterned supported lipid bilayer membranes.
J Biomed Mater Res. 2001;55:487-495.

Calvacanti-Adam EA, Micoulet A, Blçmmel J, Auernheimer J, Kessler H, Spatz JP.
Lateral spacing of integrin ligands influences cell spreading and focal adhesion assembly.
Eur J Cell Biol. 2006;85:219-224. Pubmed Link

Cherniavskaya O, Chen C, Heller E, Sun E, Provezano J, Kam L, Hone J, Sheetz MP, Wind SJ.
Fabrication and surface chemistry of nanoscale bioarrays for the study of cytoskeletal protein binding interactions and their effect on cell motility.
J Vac Sci Technol. 2005;B23:2972.

Ma'ayan A, Jenkins SL, Neves S, Hasseldine A, Grace E, Dubin-Thaler B, Eungdamrong NJ, Weng G, Ram PT, Rice JJ, Kershenbaum A, Stolovitzky GA, Blitzer RD, Iyengar R.
Formation of regulatory patterns during signal propagation in a mammalian cellular network.
Science. 2005;309(5737):1078-1083.

Balaban NQ, Schwartz US, Riveline D, Goichberg P, Tzur G, Sabanay I, Mahalu D, Safran S, Bershadsky A, Addadi L, Geiger B.
Force at single focal adhesions determines their assembly: a study using elastic micro-patterned substrates.
Nat Cell Biol. 2001;3: 466-472.

Bershadsky AD, Balaban NQ, Geiger B.
Adhesion-dependent cell mechanosensitivity.
Annu Rev Cell Dev Biol. 2003;19:677-695.

Zaidel-Bar R, Ballestrem C, Kam Z, Geiger B.
Early molecular events in the assembly of matrix adhesions at the leading edge of migrating cells.
J Cell Sci. 2003;116(22):4605-4613. Pubmed Link

Mossman KD, Campi G, Groves JT, Dustin ML.
Altered TCR signaling from geometrically repatterned immunological synapses.
Science. 2005;310:1191-1193.

Grakoui A, Bromley SK, Sumen C, Davis MM, Shaw AS, Allen PM, Dustin ML.
The immunological synapse: a molecular machine controlling T cell activation.
Science. 1999;285:221-227.

Campi G, Varma R, Dustin ML.
Actin and agonist MHC-peptide complex-dependent T cell receptor microclusters as scaffolds for signaling.
J Exp Med. 2005;202:1031-1036.

Vogel V.
Mechanotransduction involving multimodular proteins: converting force into biochemical signals.
Annu Rev Biophys Biomol Struct. 2006;35:459-488.

Thomas WE, Forero M, Yakovenko O, Nilsson L, Vicini P, Sokurenko E, Vogel V.
Catch bond model derived from allostery explains force-activated bacterial adhesion.
Biophys J. 2005;90:753-764. Pubmed Link

Puklin-Faucher E, Gao M, Schulten K, Vogel V.
How the headpiece hinge angle is opened: new insights into the dynamics of integrin activation.
J Cell Biol.